1. Assertion (A): Almost all enzymes are proteins.
Reason (R): Most of the enzymes are polymers of amino acids.
A: A and R are correct. R explains A.
2. First name of enzyme
A: Ferments
3. The term enzyme was coined by
A: Willy Kuhne
4. Match the following.
| List - I | List - II |
| I) Enzyme | A) First discovered enzyme |
| II) Precursor of enzyme | B) Louis Pasteur |
| III) Zymase | C) Zymogen |
| IV) Ferments | D) Protein |
Correct matching is
I II III IV
A: D C A B
5. The term ferments was coined by
A: Louis Pasteur
6. An enzyme has
1) Primary structure 2) Tertiary structure
3) Secondary structure 4) All
A: 4(All)
7. The similarity between enzymes and inorganic catalysts is that both are
A: Concerned with bringing change in the velocity of a chemical reaction without under going any permanent change
8. Assertion (A): Enzymes are universal.
Reason (R): They are present in all organisms which show sexuality.
A: A and R are correct. R explains A.
9. The definition of enzyme was given by
A: Dixon, Web
10. The source of first enzyme discovered is
A: Yeast
11. Assertion (A): All enzymes are proteins.
Reason (R): Nucleic acid like 23s rRNA can act as enzyme.
A: A is false. R is correct.
12. Assertion (A): Enzyme is a polymer of amino acids.
Reason (R): Chemically enzymes are proteins.
A: A and R are correct. R explains A.
13. Find out mismatch.
1) First essay on enzyme - J.B.S. Haldane
2) First enzyme crystallised - Urease
3) First enzyme discovered - Zymase
4) First name of the enzyme - Zymogen
A: 4(First name of the enzyme - Zymogen)
14. Match the following.
| List - I | List - II |
| I) Enzyme | A) Polymer of Amino acids |
| II) Ribozyme | B) Chromogenic substrate |
| III) Taq polymerase | C) RNA |
| IV) β − Galactosidase | D) Thermostable |
Correct matching is
I II III IV
A: A C D B
15. A functional enzyme shows
A: Tertiary structure
16. Generally enzyme gets damaged at this temperature
A: Above 40oC
17. Read the following statements and find out incorrect one.
1) Enzymes are hydrophilic colloidals.
2) Water becomes vapour. It is a physical reaction.
3) When bonds are broken and new bonds are formed, it is a chemical reaction.
4) None
A: 4(None)
18. Number of carbonic acid molecules formed from water and CO2 in the presence and absence
of carbonic anhydrase respectively are
A: 21,60,000, 200 per hour
19. Substrate + Enzyme give
A: Product + Enzyme
20. Substrate occupies into the active site of enzyme by the following process.
A: Diffusion
21. Read the following statements and find out incorrect one.
1) Acleft or pocket in the 3-D structure of a catalytic protein into which substrate fits is
called as active site.
2) A protein is an enzyme.
3) Enzymes are macromolecules.
4) Enzymes are colloidal.
A: 2(A protein is an enzyme.)
22. Read the following statements.
1) Same substrate can undergo different types of reactions and each catalysed by a
separate enzyme.
2) The last step in enzyme action is the release of product from enzyme.
3) Between substrate and product unstable intermediate structural states are formed.
4) Enzyme reduce the energy for biochemical reaction.
A: 4(Enzyme reduce the energy for biochemical reaction.)
23. If E = Enzyme, S = Substrate and P = Product. Choose the following equation for action
of enzyme on the substrate to form a product
A: E + P → [ES] → [EP] → E + S
24. Formation of (ES) complex was first explained by
A: Emil Fisher
25. Geometry of the enzyme is altered in the hypothesis of
A: Daniel E. Koshland
26. Tertiary structure of enzyme is altered by the factors like (A) substrate concentration
(B) pH (C) temperature. The following graph is suitable to X = ?
A: BC
27. Assertion (A): High temperature destroys enzymatic activity.
Reason (R): Low temperature preserves the enzyme in a temporarily inactive state.
A: A and R are correct. R do not explains A.
28. Km value is
1) 
2) Approximate inverse measures of affinity of the enzyme for a given substrate
3) Michaelis - Menten constant
4) All
A: 4(All)
29. Malonate is similar to
A: Succinic acid
30. Competitive inhibitors which occupy active site on enzyme surface
A: Competitive inhibitors
31. Physically enzymes are
A: Colloidals
32. Assertion (A): Enzymes are heat sensitive.
Reason (R): They are colloidals.
A: A and R are correct. R explains A.
33. Assertion (A): Enzymes are almost inactive at lower temperature.
Reason (R): They are denatured at lower temperature.
A: A is correct. R is false.
34. Protein nature of the enzyme was first established by
A: Sumner
35. Match the following.
| List − I | List − II |
| I) Optimum temperature | A) 25 − 30oC |
| II) Low temperature | B) Denatured |
| III) High temperature | C) Sensitive |
| IV) Heat | D) Less active |
A B C D
A: I III IV II
36. Assertion (A): Enzymes speed up the rate of a reaction.
Reason (R): Enzymes change the equilibrium of a reaction.
A: A is correct. R is false.
37. Assertion (A): Food kept in refrigerator is not spoiled.
Reason (R): Very cold temperature keeps the enzyme in a permanently inactive state
A: A is correct. R is false.
38. Read the following statements and find out incorrect one.
1) Enzymes are specific in action.
2) Generally enzymes are polymers.
3) Most of the enzymes work at neutral pH.
4) All enzymes are reversible in their action.
A: 4(All enzymes are reversible in their action.)
39. Co-enzymes are
1) metals 2) small organic molecules
3) small inorganic molecules 4) large inorganic molecules
A: small organic molecules
40. The following are regenerated
A: Coenzyme
41. Enzymes are
A: hydrophilic
42. The cell free enzyme that participates in fermentation is
A: Zymase
43. Match the following.
| List − I | List − II |
| I) Haem | A) Carboxylase |
| II) Niacin | B) Catalase |
| III) Zinc | C) IAA Oxidase |
| IV) Mn | D) NADP |
| E) Peptidase |
The correct matching is
I II III IV
A: B D A C
44. Match the following.
| List − I | List − II |
| I) Peroxidase | A) Molybdenum |
| II) Carboxy peptidase | B) Haem |
| III) Urease | C) Nickel |
| IV) Nitrogenase | D) Zinc |
| E) Copper |
The correct matching is
I II III IV
A: B D C A
45. Metal ions of the enzymes are concerned with
A. To form co-ordination bonds with side chains at the active site.
B. To form one or more co-ordination bonds with the substrate.
1) Neither A nor B 2) B 3) A 4) A and B
A: A and B
46. Protein part of the holoenzyme is called
A: Apoenzyme
47. Assertion (A): Enzymes enhance the rate of the reaction.
Reason (R): Enzymes lower the activation energy.
A: A and R are correct. R do not explains A
48. Match the following.
| List − I | List − II |
| I) Cofactor | A) Polymer of amino acids |
| II) Coenzyme | B) Non protein constituent of holoenzyme |
| III) Apoenzyme | C) Organic compound tightly bound to apoenzyme |
| IV) Prosthetic | D) Organic compounds transiently associated with Apoenzyme. |
Correct matching is
I II III IV
A: B D A C
49. Assertion (A): Coenzymes are cofactors.
Reason (R): All cofactors are not prosthetic groups.
A: A and R are correct. R do not explains A.
50. Prosthetic group is a
A: Part of active site of holoenzyme
51. Turn Over Number (TON) indicates
Ans: Number of substrate molecules converted to products.
52. Turn Over Number indicates
Ans: nature of substrate
53. To calculate the turn over number of an enzyme the following data is not necessary
1) Number of products 2) Number of substrate molecules
3) Time taken 4) Moles of enzymes
Ans: Number of products
54. The following is a vitamin
1) NADP 2) FAD 3) NAD 4)Niacin
Ans: Niacin
55. The E.C. of Glucose-6-Phosphotransferase is
Ans: 2.7.1.2
56. The first number in E.C. must be
Ans: less than 7
57. The second number in E.C. is always
1) above 3 2) less than 13 3) less than 14 4) 1, 3
Ans: 4 (1, 3)
58. Enzyme code is
1) 4 digit number 2) Decided by I.U.B. 3) Permanent 4)All
Ans: 4 (All)
59. Read the following statements and find out incorrect one.
1) All the enzymes discovered so far are divided into 6 classes.
2) IUB means International Union of Biochemistry.
3) Enzyme classification is based on the type of chemical reactions they catalyse.
4) Competitive inhibitor closely resembles the substrate in its molecular structure.
Ans: 4 (All the enzymes discovered so far are divided into 6 classes.)
60. Malonate resembles
Ans: Succinate
61. Assertion (A): Catalytic activity is lost when cofactor is removed from the enzyme.
Reason (R): Cofactor is non-protein part of the enzyme.
Ans: A and R are correct. R do not explains A.
